OBJECTIVE To establish a HPLC-DAD analysis method for simultaneous determination of ten hy-polipidemic drugs,and a two-dimensional qualitative database was established based on the retention time and ultra-violet absorption spectrum of various substances.This database was used for rapid screening of illegal addition in hy-polipidemic products.METHODS The gradient elution was used with 0.5％formic acid as mobile phase A and acetonitrile as mobile phase B.The retention time and ultraviolet absorption spectra of 10 reference materials were collected,and a two-dimensional qualitative database was established on this basis.RESULTS Based on the HPLC-DAD method,a new database for rapid screening of illegal addition of lipid-lowering products was established in this experiment.The results were accurate and reliable through the detection of market sampling samples and simulated positive samples.CONCLUSION The HPLC-DAD database established in this experiment can quickly screen the illegally added chemical drugs in products.This method is simple and fast,and provides a reliable methodological ref-erence for the basic inspection institutions.
维普
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