Separation and Preparation of Tetrandrine and Fangchinoline from Stephania tetrandra by High-Speed Countercurrent Chromatography
OBJECTIVE The main active components of Stephania tetrandra are tetrandrine and fangchino-line.This paper aims at obtaining a rapid separation and preparation method of tetrandrine and fangchinoline from Stephania tetrandra.METHODS The distribution coefficients of tetrandrine and fangchinoline in the upper and lower phases of mixture solvents were determined by extraction using different mixture solvents.The two alkaloids were separated and prepared by high-speed countercurrent chromatography(HSCCC)and their purities were also tested.RESULTS The optimized solvent system of n-hexane-ethyl acetate-methanol-water-triethylamine(7∶3∶5∶4∶1)was obtained,which meet the experimental requirement.Afterwards,fangchinoline(F1)and tetrandrine(F2)were obtained by continuously separation within 70 min by HSCCC using upper phase as the stationary phase and the lower phase as the mobile phase at a flow rate of 3 mL·min-1.The purities of fangchinoline(F1)and tetrandrine(F2)were determined to be 97％and 95％,respectively.CONCLUSION The present study provided a rapid preparation of tetrandrine and fangchinoline by HSCCC.This is time-saving method in comparison of the existing methods such as column chromatography and pH gradient countercurrent chromatography.
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