Studies on the Antibacterial Activity of Ai ChangXiang Essential Oil
OBJECTIVE The antibacterial activity of Ai ChangXiang essential oil was studied to provide theo-retical support for the further application and utilization of the essential oil of traditional Chinese herbal formu-la.METHODS The essential oil was extracted from the traditional Chinese medicine crude powder by steam distil-lation and the specific weight and extracted yield were detected too.We tested the sensitivities of Escherichia Coli,Staphylococcus aureus,drug-resistant Staphylococcus aureus,Pseudomonas aeruginosa,Salmonella typhi and Bacillus paratyphosus to Ai ChangXiang essential oil using the inhibition zone test.Additionally,we determined the minimum inhibitory concentration(MIC)of Ai ChangXiang essential oil against these 6 species of bacteria using broth microdi-lution method to assess its antibacterial effect.RESULTS The ρ=0.94 g/cm3 and extracted yield was 1.30％.The size of the inhibition zone of essential oil against E.Coli,S.aureus,drug-resistant S.aureus,P.aeruginosa,S.typhi and B.paratyphosus were 27.0 mm,26.0 mm,25.7 mm,10 mm,25.7 mm and 22.3 mm,and the corresponding MIC were 1.175 mg·mL-1,2.350 mg·mL-1,2.350 mg·mL-1,9.400 mg·mL-1,0.588 mg·mL-1 and 2.350 mg·mL-1,respectively.CONCLUSION The efficacy of Ai ChangXiang essential oil against 6 species of bacteria was demonstrated,as well as its antibacterial effect on S.typhi and E.Coli was the best.
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