HPLC法测定蔬菜中α-胡萝卜素和β-胡萝卜素的前处理方法优化
Optimization of Pretreatment Methods for the Determination of α-carotene and β-carotene in Vegetables by HPLC Method
郑良珺 1陈倩 2郑仁锦3
作者信息
- 1. 泉州医学高等专科学校,福建泉州 362046
- 2. 福建医科大学药学院,福建 福州 350000
- 3. 福建省疾病预防控制中心,福建 福州 350000
- 折叠
摘要
目的 优化HPLC法测定蔬菜中α-胡萝卜素和β-胡萝卜素的前处理方法,以提高现有测定方法的检测效率.方法 本研究以国标GB 5009.83-2016为基础,通过优化皂化条件、酸化法代替水洗法等,改进蔬菜中α-胡萝卜素和β-胡萝卜素测定的前处理方法.结果 本研究利用酸化法改良后,方法的平均加标回收率为86.9％～98.7％,相对标准偏差为3.2％～5.4％,在0.5～10 μg·mL-1线性范围内线性良好.结论 本研究通过优化HPLC的前处理方法,显著提高了蔬菜中胡萝卜素的检测效率,节省了人力与试剂成本,为胡萝卜素检测技术的改良提供了创新性的思路与方向,可广泛应用于蔬菜的品质检测与营养评估.
Abstract
OBJECTIVE To optimize the pretreatment methods for the determination of α-carotene and β-caro-tene in vegetables by HPLC in order to improve the detection efficiency of the existing determination meth-ods.METHODS In this study,the pretreatment methods for α-carotene and β-carotene determination in vegetables were improved by optimizing the saponification conditions and the acidification method instead of water washing meth-od based on the national standard GB 5009.83-2016.RESULTS The average spiked recoveries of the optimized method with acidification in this study were 86.9％-98.7％with the relative standard deviations(RSDs)of 3.2％-5.4％,and the linearities were good in the linear range of 0.5-10 μg·mL-1.CONCLUSION By optimizing the pretreatment methods of HPLC,this study significantly improved the detection efficiency of α-carotene and β-carotene in vegetables,saved labor and reagent costs,and provided innovative ideas and directions for the improvement of carote-noid detection technology,which can be widely applied to the quality testing and nutritional assessment of vegetables.
关键词
高效液相色谱法/蔬菜/α-胡萝卜素/β-胡萝卜素/方法优化Key words
HPLC/Vegetables/α-carotene/β-carotene/Method Optimization引用本文复制引用
基金项目
福建省卫健委中青年骨干项目(2023GGA040)
出版年
2024
NETL
NSTL
万方数据