Simultaneous Determination of 5 Components in YiGanLing Capsules by HPLC-DAD
OBJECTIVE To establish a high performance liquid chromatography-diode array detection(HPLC-DAD)method for simultaneous determination of five main components in YiGanLing Capsules.METHODS The mobile phase was acetonitrile-0.05％phosphoric acid aqueous solution,the chromatographic column was C18,the de-tection wavelength was 232 nm(paeoniflorin detection),258 nm(emodin,emodin,chrysophanol detection),323 nm(chlorogenic acid detection).RESULTS The linear relationship of the method was good(R≥0.995),and the precision RSD was less than 2％.CONCLUSION The method is efficient and convenient,and can be used for the quality control of YiGanLing Capsules.
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