OBJECTIVE To study the in vitro anti-inflammatory activity and mechanism of ferulic acid.METHODS Establishing the in vitro M2 type macrophages inflammation model under the suitable condi-tion.CCK-8 method was used to detect the activity of M2 macrophages.Flow cytometry was used to detect the polari-zation of M2 macrophages.ELISA and real-time quantitative PCR were used to detect the mRNA and protein expres-sion of transforming growth factor-β1(TGF-β1)and interleukin-10(IL-10)by M2 type macrophages.Western blot was used to detect the expression of p38 MAPK and NF-κB p65.RESULTS Under ferulic acid treatment,the ex-pression of M2 type macrophage marker CD206+was significantly decreased,the expression of TGF-β1,IL-10,p-p38 MAPK and p-NF-κB p65 on M2 type macrophages were significantly promoted in a dose-dependent man-ner.CONCLUSION Ferulic acid can promote the polarization of M0 macrophages towards M2 type and promote the expression of anti-inflammatory factors,which is related to the promotion of p38 MAPK and NF-κB signaling pathway.
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