Determination of Residual Ethanol and Chloroform in XiaoEr HuaTan ZhiKe Syrup
OBJECTIVE To establish a headspace GC method for determination of ethanol and chloroform in XiaoEr HuaTan ZhiKe Syrup.METHODS A DB-624(SN:USR593111J)column was used with nitrogen as the carrier gas.Headspace injection was applied with the injection port temperature at 200 ℃.The column temperature program was set as:remaining the starting temperature of 60 ℃ for 8 min,then increasing to 120 ℃ at the rate of 8℃/mnin and remaining 120℃ for 15 min.The detector temperature was 250 ℃,the headspace equilibrium tempera-ture was 80 ℃,and the equilibration time was 30 min,the injection split was 10∶1.An FID was uesd as the detector with the temperature at 250 ℃.RESULTS The linear range of ethanol fell into 0.103-5.131 mg(r=0.999)and chloroform fell into 0.57-28.65 μg(r=0.999);The RSD of precision were 1.02％and 1.26％;and the RSD of re-peatability were 1.86％and 1.97％;The limits of quantitation were 1.72 µg and 30.86 µg;The average recoveries were 102％and 99％(n=6,RSD were 1.83％and 4.52％).Six batches of ethanol and chloroform from two manu-facturers exceeded the limits.CONCLUSION The establisded method has the high sensitivity,good specificity and good applicability.It can be applicable to the determination of residuai ethanol and chloroform.
万方数据
维普
