The Content of Three Alkalods in ZuoJin Pills was Determined by Quanti-tative Analysis of Multi-components with Single Maker
OBJECTIVE A method was developed to determine the quality of the different types of alkaloids(berberine hydrochloride,evodiamine and rutaecarpin)by RP-HPLC and quantitative analysis of multi-components with single maker(QAMS)in ZuoJin Pills.METHODS The samples were extraced with 70％ethanol and heated to reflux in water bath.The MG C18 column(4.6 mm ×250 mm,5 μm)was used.The mobile phase was consisted of acetonitrile(A)-aqueous solution containing 0.1％phosphoric acid(B).The flow rate of the mobile phase was 1.0 mL·min-1.The column temperature was set at 35 ℃.The alkaloids were monitored at three different detection wavelengths which 264 nm for berberine hydrochloride,226 nm for evodiamine and 342 nm for rutae-carpin.RESULTS The injection volume of alkaloids displayed well linear range within the range of 8.68-173.521 µg for berberine hydrochloride,1.04-20.83 μg for evodiamine and 1.06-21.19 µg for rutaecarpin.The recoveries of three alkaloids,berberine hydrochloride,evodiamine and rutaecarpin,were 99.0％,98.8％,100.4％,respective-ly.The relative correction factor between evodiamine and berberine hydrochloride,rutaecarpin and berberine hydro-chloride were 2.053 and 1.671,respectively.The results were close to that of the external standard meth-od.CONCLUSION The method is accurate and reliable,it can be used for simultaneous determination of three al-kaloids in ZuoJin Pills.
万方数据
维普
