Determination of Hydroxychloroquine in Human Whole Blood by UPLC-MS/MS
OBJECTIVE To establish the determination method of hydroxychloroquine in human whole blood with ultra performance liquid chromatography-tandem mass spectrometry(UPLC-MS/MS).METHODS Whole blood samples were precipitated by acetonitrile,then the supernatant was determinated by UPLC-MS/MS.Column ACQUITY UPLCHSS T3(2.1 ×50 mm,1.8 μm)was used with mobile phase A of water(containing 0.3％formic and 3 mmol·L-1 ammonium formate)and mobile phase B of acetonitrile∶methanol∶water(V∶V∶V,95∶95∶10)(con-taining 0.3％formic and 3 mmol·L-1ammonium formate),with the gradient elution at a flow rate of 0.30 mL·min-1.Electrospray positive ion model and multiple reaction monitoring were used.RESULTS There was a good linerrelationship in the calibration curve of 1-500 ng·mL-1 for hydroxychloroquine,and the intra-day and inter-day relative standard deviations were less than 10％.CONCLUSION The UPLC-MS/MS method was accuracy,sensi-tive and simple,and can be applied to a pharmacokinetics of hydroxychloroquine in human.
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维普
