Reusable Magnetic Enzyme Extracted Ganoderma lucidum Polysaccha-ride and Triterpenoid Substances
OBJECTIVE To prepare a reusable magnetic nanoparticle Fe3O4 immobilized cellulase to extract polysaccharide and triterpene components in ganoderma lucidum,improve the extraction rate of polysaccharide and triterpenes and the reuse rate of cellulase.METHODS By preparing magnetic nano Fe3O4,synthesized magnetic nano Fe3O4@SiO2,and functionalized with APTES,we immobilized cellulase.The relative enzyme activity of the im-mobilized enzyme was measured for the reutilization experiment of cellulase,and the effective ingredients of Ganoder-ma lucidum were extracted by enzyme-assisted ultrasound.The content of Ganoderma lucidum polysaccharide and on-olic acid were determined by anthracenone-sulfuric acid using a UV spectrophotometer.RESULTS The relative en-zyme activity was about 100％,the polysaccharide extraction rate of Ganoderma lucidum extract was 5.3％and triter-penes was 2.0％CONCLUSION Magnetic Fe3O4 nanoparticle carrier immobilized cellulase has good reusable properties and reduces the amount of cellulase used.The use of ultrasound-assisted immobilization enzyme has effec-tively improved the extraction rate of ganoderma polysaccharide and triterpenes.
ReusabilityGanoderma lucidum polysaccharideGanoderma lucidum triterpenesFe3O4 magnetic nanoparticlesImmo-bilized cellulase
万方数据
维普
