OBJECTIVE To prepare a reusable magnetic nanoparticle Fe3O4 immobilized cellulase to extract polysaccharide and triterpene components in ganoderma lucidum,improve the extraction rate of polysaccharide and triterpenes and the reuse rate of cellulase.METHODS By preparing magnetic nano Fe3O4,synthesized magnetic nano Fe3O4@SiO2,and functionalized with APTES,we immobilized cellulase.The relative enzyme activity of the im-mobilized enzyme was measured for the reutilization experiment of cellulase,and the effective ingredients of Ganoder-ma lucidum were extracted by enzyme-assisted ultrasound.The content of Ganoderma lucidum polysaccharide and on-olic acid were determined by anthracenone-sulfuric acid using a UV spectrophotometer.RESULTS The relative en-zyme activity was about 100％,the polysaccharide extraction rate of Ganoderma lucidum extract was 5.3％and triter-penes was 2.0％CONCLUSION Magnetic Fe3O4 nanoparticle carrier immobilized cellulase has good reusable properties and reduces the amount of cellulase used.The use of ultrasound-assisted immobilization enzyme has effec-tively improved the extraction rate of ganoderma polysaccharide and triterpenes.
关键词
可重复利用性/灵芝多糖/灵芝三萜/Fe3O4磁性纳米粒子/固定化纤维素酶
Key words
Reusability/Ganoderma lucidum polysaccharide/Ganoderma lucidum triterpenes/Fe3O4 magnetic nanoparticles/Immo-bilized cellulase
维普
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