Simultaneous Determination of Five Saponins in ShuJin ZhiTong Liquid by HPLC
OBJECTIVE To establish an HPLC method for the simultaneous content determination of notogin-senoside R1,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and Ginsenoside Rd in ShuJin ZhiTong Liq-uid.METHODS SinoChrom ODS-BP(4.6 mm x250 mm,5 μm)column was used with acetonitrile and water as the mobile phase with gradient elution at a flow of 1.3 mL·min-1.The column temperature was 30 ℃ and the de-tection wavelength was 203 nm.RESULTS Notoginsenoside R1,ginsenoside Rg1,ginsenoside Re,ginsenoside Rb1 and ginsenoside Rd showed good linear relationships in the concentration range examined(r≥0.9998),the linear ranges were(24.12-482.40)μg·mL-1、(55.56-111.20)μg·mL-1、(7.30-146.00)μg·mL-1、(49.62-992.40)μg·mL-1、(34.00-680.00)μg·mL-1,whose average recoveries were 98.93％,99.61％,97.87％,99.06％,98.96％,and RSDs were 2.75％,2.43％,1.91％,2.57％,2.94％,respectively.CONCLUSION This simple and accurate method can provide the method basis for the quality control of ShuJin ZhiTong Liquid.
万方数据
维普
