The way of bioavailable heavy metals entering human body through soil is less concerned in human health risk assessment of industrial sites.The accuracy of bioavailable heavy metal content affects the reliability of human health risk assessment results.Taking a typical chemical industry site as an example,soil samples with different pollution levels were collected.Tessier sequential extraction method was used to determine the content of different forms of arsenic in soil and the biological activity coefficient of arsenic.In Vitro gastrointestinal simulation experiment was used to calculate the bio-availability of arsenic in the stomach and small intestine,and the bioavailability of arsenic was compared.The results showed that the content of residual arsenic in soil samples was the highest,while the content of available arsenic was rela-tively low,and the biological activity coefficient was 0.002 4~0.021 0.There was a linear positive correlation between the available arsenic content absorbed in the stomach and small intestine and the arsenic content in the soil.The bioavail-ability of arsenic was 0.78%~36.81%.The available arsenic content obtained by the Tessier sequential extraction method was lower than that obtained by the In Vitro method.It is suggested that in the human health risk assessment of heavy metal contaminated soil,the digestive system simulation experiment should be carried out to obtain more reliable data of available heavy metal content.