Karst water has indicative significance for maintaining ecological balance.Taking Jiuluzhai ecological tourism area in Hubei Province as the study area,based on the division of karst water aquifers,karst water samples were systemat-ically collected,piper three-line diagram,Gibbs diagram,main ion ratio,H-O isotope and Sr isotope methods were used to analyze the chemical characteristics and formation mechanism of karst water.The results show that the karst water aquifer in the study area can be generalized into three layers:Ordovician-Cambrian aquifer,Cambrian aquifer and Sinian aquifer.The karst water is a weak alkaline fresh water,and the hydrochemical type is mainly HCO3-Ca·Mg type.The δ2 H of karst water is-62.63‰ to-52.54‰,the δ18 O is-9.63‰ to-7.90‰,and the 87 Sr/86 Sr ratio is 0.708 772 to 0.725 915.The recharge source of karst water is atmospheric precipitation,and its hydrochemical components are mainly controlled by rock weathering.In the process of runoff,it has significant water-rock interaction with aquifer rock minerals(limestone is dominant,followed by dolomite and silicate).