Characteristic chromatogram of Fructus Tritici Levis formula granules and quantitative study of four nucleosides
In order to realize quality control and evaluation of Fructus Tritici Levis formula granules,a method of characteristic chromatogram of Fructus Tritici Levis formula granules and determination of four nucleosides was established by HPLC.The analysis was performed on Waters XSelect· HSS T3(4.6 mm×250 mm,5 μm)column with mobile phase consisted of methanol-water(gradient elution)at the flow rate of 1.0 mL/min.The column temperature was set at 30℃,and detection wavelength was set at 260 nm.The results show that there are 8 common peaks in 13 batches of Fructus Tritici Levis formula granules,and all of them were identified as uracil,cytidine,hypoxanthine,uridine,adenine,guanosine,tryptophan and adenosine.The RSD value of relative retention time of each common peak is less than 2.0%.Uracil,uridine,guanosine and adenosine show good linearity(r = 1.0000)in the ranges of 0.0019～0.0473 mg/mL,0.0039～0.0982 mg/mL,0.0028～0.0701 mg/mL and 0.0029～0.0730 mg/mL respectively.The average recoveries were 99.0%,100.7%,101.2%and 101.8%.The established method for the determination of nucleoside components is accurate,efficient and reproducible.It can be used for the quality control of Fructus Tritici Levis formula granules.
chemistry of Chinese materia medicaFructus Tritici Levisformula granulesHPLCcharacteristic chromato-gramnucleosidesdetermination
万方数据
维普
