Study on the preparation and quality standard of Geum japonicum Thunb.var.chinense Bolle standard decoction
Inorder to realize quality control of the standard decoction of Geum japonicum Thunb.var.chinense Bolle,a method of characteristic chromatogram of Geum japonicum Thunb.var.chinense Bolle standard decoction and determination of ellagic acid was established by HPLC.The analysis was performed on Waters Symmetry C18 column(4.6 mm × 250 mm,5.0 μm)with acetonitrile-0.1％phosphoric acid for gradient elution.The flow rate was 1.0 mL/min,the detection wavelength was 360 nm and the column temperature was 35 ℃.The results show that the average extraction rate of 15 batches of Geum japonicum Thunb.var.chinense Bolle standard decoction is 17.8％;The characteristic chromatogram similarity is 0.793～1.000 with a total of 13 common characteristic peaks calibrated and four components confirmed,namely caffeic acid(peak 4),ellagic acid(peak 8),isoquercitrin(peak 9),and luteolin(peak 10);The average transfer rate of ellagic acid from Geum japonicum Thunb.var.chinense Bolle to its standard decoction is 45.90％,indicating that the quality of the standard decoction has been well controlled.The preparation process of the proposed Geum japonicum Thunb.var.chinense Bolle standard decoction is feasible,and the established characteristic chromatogram and content determination method of standard decoction are stable,which can lay a foundation for the quality standard research of the formulation granule of Geum japonicum Thunb.var.chinense Bolle standard decoction.
chemistry of Chinese materia medicaGeum japonicum Thunb.var.chinense Bollestandard decoctioncharac-teristic chromatogramcontent determination
万方数据
维普
