Determination of Total Fiavonoids Content in Melilotus by Spectrophotometry
The content of total flavonoids in Melilotus was determined by spectrophotometry. Rutin was used as the standard sample and NaNO2-Al(NO3)3-NaOH was used to compose the chromogenic system. The detection wavelength was set at 510 nm. A good linear relationship was observed in the range of 10-50 μg · mL-1 ($ = 0. 9992) and the average recovery rate was 99. 19% with a relative standard deviation of 0. 79%(rt= 9). The average content of total flavonoids in Melilotus was 1. 44%. This method is simple,reliable,accurate and rapid with good reproducibility and stability. It is a suitable way for determination of total flavonoids in Melilotus.
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NSTL
万方数据
维普
