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无花果多糖脱色工艺研究

Decoloring Process of Polysaccharide from Ficus carica L.

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采用大孔吸附树脂优化无花果多糖的脱色工艺.以无花果干果为原料,热水浸提得无花果多糖粗提物,经Sevage试剂去蛋白、乙醇沉淀后冻干得粗多糖粉末.以脱色率和多糖保留率为考察指标,筛选适合无花果多糖脱色的最优树脂和最佳的脱色工艺条件.结果表明,无花果多糖脱色的最优树脂为LSD-296,最佳脱色工艺条件为:树脂用量2.0 g、多糖溶液浓度4 mg·mL-1、pH值6、脱色温度40℃,为无花果多糖进一步分离纯化奠定了基础.
We optimized the decoloring process of the polysaccharide from Ficus carica L. by macroporous resin. U-sing the dry fruit of Ficus carica L. as a raw material,we prepared the crude polysaccharide from Ficus carica L. by hot water extraction,and obtained crude polysaccharide powder using freeze-dry method after protein removal by Sevage rea-gent and ethanol precipitation. Using decolorization rate and polysaccharide retention rate as indexes,we screened the op-timal resin and the optimal conditions of decoloring process as follows:choosing LSD-296 as the optimal resin,the dosage of resin of 2. 0 g,the concentration of polysaccharide solution of 4 mg·mL-1 ,pH value of 6,and the decoloring tempera-ture of 40 ℃. This study provides the basis for the separation and purification of polysaccharide from Ficus carica L. .

polysaccharide from Ficus carica L.decoloring processmacroporous resin

祖思佳、倪孟祥

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中国药科大学生命科学与技术学院,江苏 南京210009

无花果多糖 脱色工艺 大孔树脂

2017

化学与生物工程
武汉工程大学 湖北省化学化工学会 湖北省化学研究院 湖北省化学工业研究设计院

化学与生物工程

CSTPCD
影响因子:0.351
ISSN:1672-5425
年,卷(期):2017.34(9)
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