Antioxidant Activity of Osmanthus fragrans Extract and Its Protection Effect on t-BHP-Induced Oxidative Damage of HepG2 Cells
We evaluated the antioxidant activities of water extract,95％ethanol extract,n-butanol extract,ethyl acetate extract,and petroleum ether extract from Osmanthus fragrans by DPPH method,ABTS method,and FRAP method.Moreover,we investigated the protective effects of water extract from Osmanthus fragrans on tert-butyl hydroperoxide(t-BHP)-induced oxidative damage of human hepatocellular carcinoma cells HepG2.The results show that the Osmanthus fragrans extracts have certain antioxidant activities,but the an-tioxidant activities of different solvent extracts from Osmanthus fragrans are different.The water extract from Osmanthus fragrans has good biocompatibility with HepG2 cells,which can inhibit the production of 8-hydrox-y-2'-deoxyguanosine(8-OHdG),tumor necrosis factor-α(TNF-α),and interleukin-1β(IL-1β)in HepG2 cells in-duced by t-BHP,and also significantly reduce the level of malondialdehyde(MDA).
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