首页|基于双指标序列分析法和聚类分析法的洋甘菊红外指纹图谱研究

基于双指标序列分析法和聚类分析法的洋甘菊红外指纹图谱研究

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采用傅立叶变换红外光谱仪采集了不同温度、不同部位、不同批次洋甘菊药材的红外光谱及二阶导数红外光谱,并结合双指标序列分析法、聚类分析法对不同批次洋甘菊药材进行了全面评价。结果表明,从洋甘菊红外光谱中确定了 19个共有峰,根据不同温度下各特征吸收峰的变化,推测温度可能影响洋甘菊中黄酮类、酯类、糖(苷)类物质;洋甘菊花、茎的红外光谱具有一定的相似性和差异性,根据特征吸收峰强度推测花中黄酮类、酚酸类含量较高;不同批次洋甘菊的峰位、峰强度存在一定相似性和差异性,二阶导数红外光谱可以分离出红外光谱中一些相互重叠的吸收峰;不同批次洋甘菊样品间共有峰率为60。00%~100。00%,变异峰率为0。00~47。62%;当欧氏距离为5~10时,16批次洋甘菊药材可聚为 3 类,其中 S1、S4、S5、S6、S7、S10、S12 聚为一类,S3、S8、S9、S11、S13、S15、S16 聚为一类,S2、S14 聚为一类。该方法通过不同角度对洋甘菊药材的差异进行分析评价,具有一定的可行性,为药材的质量控制提供了新方法。
Infrared Fingerprint Spectra Analysis of Matricaria chamomilla L.Based on Dual-Index Sequence Analysis and Cluster Analysis
We collected infrared spectra and second derivative infrared spectra of Matricaria chamomilla L.from different temperatures,different parts,and different batches by Fourier transform infrared spectroscopy,and evaluated different batches of Matricaria chamomilla L.by infrared spectra and second derivative infrared spectra combined with dual-index sequence analysis and cluster analysis.The results show that 19 common peaks are identified from the infrared spectra of Matricaria chamomilla L.,and the characteristic peaks of samples vary under different temperatures,indicating that temperature may affect flavonoids,esters,and glyco-sides from Matricaria chamomilla L..The infrared spectra of flowers and stems of Matricaria chamomilla L.have certain similarities and differences.According to the intensity of characteristic absorption peak,it is specu-lated that the contents of flavonoids and phenolic acids in flowers are higher.The peaks and intensities of differ-ent batches of Matricaria chamomilla L.have certain similarities and differences.The second derivative infra-red spectra can separate some overlapping absorption peaks in the infrared spectra.The common peak rates of different batches of Matricaria chamomilla L.samples are 60.00%-100.00%,and the variant peak rates are 0.00-47.62%.When the Euclidean distance is 5-10,sixteen batches of Matricaria chamomilla L.can be clustered into 3 categories,among which S1,S4,S5,S6,S7,S10,and S12 are clustered into one category;S3,S8,S9,S11,S13,S15,and S16 are grouped into one category;S2 and S14 are categorized into one category.This method analyzes and evaluates the differences of Matricaria chamomilla L.herbs from different angles,which has certain feasibility and provides a new method for the quality control of medicinal herbs.

Matricaria chamomilla L.infrared spectrumdual-index sequence analysiscluster analysis

杨青青、刘婷、张明惠、马璇、姚军

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新奇康药业股份有限公司,新疆乌鲁木齐 830011

新疆医科大学药学院,新疆乌鲁木齐,830054

新疆奇沐医药研究院,新疆乌鲁木齐,830011

新疆中药(民族药)制药共性关键技术研究重点实验室,新疆 乌鲁木齐 830011

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洋甘菊 红外光谱 双指标序列分析法 聚类分析法

2025

化学与生物工程
武汉工程大学 湖北省化学化工学会 湖北省化学研究院 湖北省化学工业研究设计院

化学与生物工程

北大核心
影响因子:0.351
ISSN:1672-5425
年,卷(期):2025.42(1)