以沧州泊头市鸭梨为试验材料,对鸭梨ISSR-PCR反应体系中的模板DNA用量、dNTPs浓度、引物浓度以及Taq酶浓度进行了探索,初步建立适合鸭梨ISSR-PCR反应体系为:在20μL反应体系中,含1×TaqPCRBuffer[1.5 mmol/L Mg2+]、0.15 mmol/L dNTPs、0.40 μmol/L引物、40ng DNA和l U Taq酶.
Establishment of ISSR - PCR reaction system of Pyrus bretschneideri Rehd. cv. Yali
The main influential factors of ISSR- PCR reaction system in Yali pear of Botou were researched. The effect of different concentrations of template DNA, primer, dNTPs and Taq DNA polymerase was analyzed. The results showed that the most suitable ISSR- PCR reaction system was 1 × Taq PCR Buffer 1.5 mmol/L Mg2+ ,0.15 mmol/L dNTPs,0.4 μmol/L primer 40ng DNA template, 1.0 U Taq DNA polymerase in 20 μL reaction system.
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万方数据
维普
