Effects of different treatments on seed germination and seedling growth of Isatis indigotica Fort.
The effects of KNO3 、KMnO4 、GA3 and PEG treatments with the different concentrations under indoor culture on seed germination and seedling growth of Isatis indigotica Fort.were studied to explore effective ways to promote the seed germination and seedling growth,and to provide a theoretical basis for large-scale cultivation and production of Isatis indigotica Fort.The results showed that the root length of the seedling was siginificantly increased by 61.35% treated with 2 g/L KNO3.The water retention was siginificantly improved by 81.58% treated with 10 g/L KMnO4.The root activity of seedings was significantly increased by 17.88%,21.85% and 13.82% treated with three concentrations of GA3,respectively.The germination potential and germination rate were significantly increased by38.90% and 79.18% by treatment of 0.2 g/L GA3.The fresh weight and the seedling length were significantly improved by 84.38% and 24.24% by 0.2 g/L GA3 treatment.Theroot activity was significantly enhanced by 14.08%,19.32% and 13.57%,and the fresh weight was significantly enhanced by 75.00%,106.25% and 87.50% respectively,treated with three concentrations of PEG.The germination rate was significantly improved by 68.15%and 46.93% with 200 g/L PEG and 300 g/L PEG treatment respectively.In conclusion,the treatment of 0.2 g/L GA3 was the best,which could significantly increase the germination rate and germination potential and promote the seedling growth.