首页|60Co-γ辐射对2种锦带幼苗生长及生理指标的影响

60Co-γ辐射对2种锦带幼苗生长及生理指标的影响

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为了探究辐射诱变对"白花锦带花"和"锦带花"形态及生理特性的影响,采用不同剂量(0~300 Gy)的60Co-γ射线辐射"白花锦带花"与"锦带花"休眠期种子,观测辐射对锦带花生长的影响,测定其生长量及幼苗叶片的叶绿素含量、叶绿素荧光指标、光合作用参数、SOD、POD、MDA和可溶性蛋白含量.研究结果显示,由于辐射剂量的增大,苗高、叶长、叶宽、节间距、叶绿素荧光、光合作用参数、SOD活性、POD活性出现了明显的先升高后降低的趋势,在50 Gy时到达了峰值.同时,随着辐射剂量的增加,叶绿素含量和可溶性蛋白含量呈现出下降的趋势,MDA含量明显升高.综上所述,在(2 Gy/min)剂量率的辐射条件下"白花锦带花"种子较适宜的诱变剂量为50 Gy,而"锦带花"以50~100 Gy最合适.
Effects of 60Co-γ radiation on growth and physiological index of two kinds of Weigela florida seedlings
In order to explore the effects of radiation mutagenesis on the morphological and physiological characteristics of Weigela florida"Alba"and Weigela florida,the 60Co-γ ra-diation at different doses(0~300 Gy)was used to irradiate the dormant seeds of W.florida"Alba"and W.florida to observe the effect of radiation on the growth of alum root,the growth amount and chlorophyll content,chlorophyll fluorescence,photosynthetic index,SOD,POD,MDA and soluble protein content of seedling leaves were measured.The results showed that due to the increase of radiation dose,seedling height,leaf length,leaf width,pitch spacing,chlorophyll fluorescence,photosynthesis parameters,SOD activity and POD activity showed a clear trend of first increasing and then decreasing,reaching a peak at 50 Gy.At the same time,with the increase of radiation dose,the chlorophyll content and solu-ble protein content showed a downward trend.The MDA content was significantly in-creased.In summary,under the radiation condition of(2 Gy/min)dose rate,the mutagenic dose of W.florida"Alba"seeds was 50 Gy,the W.florida in 50-100 Gy was the most suitable.

Weigela florida"Alba"Weigela floridaCo-γ radiationmutagenic breeding

张洁、宋佳宝、史宝胜

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河北农业大学园林与旅游学院,河北保定 071000

白花锦带花 锦带花 60Co-γ射线 辐射育种

国家林业与草原局项目国家林业与草原局项目国家林业与草原局项目乡村振兴技术创新专项

KJZXSA2018005KJZXSA2019003KJZXSA20200422326511D

2024

林业与生态科学
河北农业大学

林业与生态科学

CSTPCD
影响因子:0.299
ISSN:2096-4749
年,卷(期):2024.39(1)
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