转基因107杨抗虫性研究
Study on insect resistance of transgenic Populus 107
王利峰 1陈志军 2许强 1沈建茹 1赵亚楠 1刘军侠3
作者信息
- 1. 河北农业大学林学院,河北保定 071000
- 2. 滦南县国营林场河北 唐山 063500
- 3. 河北农业大学林学院,河北保定 071000;河北省林木种质资源与森林保护重点实验室,河北保定 071000
- 折叠
摘要
为研究转基因107杨对靶标昆虫柳蓝叶甲的抗虫性以及对非靶标昆虫白杨毛蚜及天敌昆虫异色瓢虫生长发育的影响,以室内饲养的方法,比较了取食转基因107杨及对照的柳蓝叶甲各龄期幼虫的死亡率、研究了取食转基因107杨及对照107杨叶片的白杨毛蚜生长发育情况,以及取食不同株系107杨上白杨毛蚜和柳蓝叶甲的异色瓢虫幼虫的生长发育情况.结果表明,转基因107杨对靶标昆虫柳蓝叶甲幼虫表达高抗虫性;取食转基因107杨叶片的白杨毛蚜幼虫期发育时间以及生命参数与对照相比均无显著差异;取食转基因107杨上白杨毛蚜的异色瓢虫幼虫发育历期及体长体宽与对照相比无显著差异;取食转基因107杨上柳蓝叶甲的异色瓢虫幼虫发育历期及体长体宽与对照相比无显著差异.研究证明,转基因107杨对非靶标昆虫白杨毛蚜和天敌昆虫异色瓢虫生长发育无显著影响,转基因107杨生态安全性较高.
Abstract
In order to study the insect resistance of transgenic Populus 107 to the target in-sect,Plagiodera versicolora,as well as the effects on the growth and development of non-target insect,Chaitophorus populeti,and natural enemy insect,Harmonia axyridis.Provi-ding theoretical basis for the ecological safety of transgenic Populus 107,we compared the mortality rate of P.versicolora larvae at each age feeding on transgenic Populus 107 and con-trol,and studied the growth and development of C.populeti between the transgenic Populus 107 group and control group.Meanwhile,we analyzed the growth and development of H.axyridis larvae feeding on P.versicolora and C.populeti on transgenic Populus 107 and con-trol by laboratory experiments.The results showed that the transgenic Populus 107 ex-pressed high insect resistance to the target insect,P.versicolora larvae.There were no sig-nificant differences in the development time and life parameter of C.populeti larvae feeding on the leaves of the transgenic Populus 107 compared with the control.There were no sig-nificant differences in the development time as well as the body length and width of H.axyridis larvae preying on either C.populeti or P.versicolora fed on the transgenic Populus 107 compared with the control.In conclusion,this study demonstrated that the transgenic Populus 107 had no significant effect on the growth and development of the non-target insect C.populeti and the natural enemy insect H.axyridis,and the ecological safety of the trans-genic Populus 107 was high.
关键词
转基因107杨/白杨毛蚜/异色瓢虫/生态安全性Key words
transgenic Populus 107/Chaitophorus populeti/Harmonia axyridis/ecological security引用本文复制引用
基金项目
国家林业和草原局科技发展中心项目(KJZXSA202104)
国家林业和草原局科技发展中心项目(KJZXSA202001)
出版年
2024
国家科技期刊平台
NSTL
万方数据