首页|环糊精强化镰刀菌修复土壤污染生态环境中高环多环芳烃的研究

环糊精强化镰刀菌修复土壤污染生态环境中高环多环芳烃的研究

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以煤矿区高环芳烃(HMW-PAHs)污染土壤为研究对象,通过添加环糊精且接种镰刀菌ZH-H2试验,研究镰刀菌ZH-H2处理、环糊精处理及二者组合处理对污染老化土壤5环和6环6种单体PAHs的修复效果.结果表明,镰刀菌单一处理对5环和6环PAHs的去除率在14.37%~22.85%之间;在不同剂量环糊精处理下(J1、J2、J3),J2处理(1 g/kg)对6种HMW-PAHs单体的去除率表现最高,为13.51%~28.43%;在ZH-H2—环糊精2因素处理中,H+J3处理对6种HMW-PAHs单体(BbF、BkF、BaP、DbA、InP、BghiP)及总量的去除效果最佳,其去除率分别为 24.61%、25.15%、23.14%、34.18%、25.76%、23.46%、25.29%,比 ZH-H2 单一处理分别提高了 71.33%、66.68%、28.94%、49.58%、44.94%、55.88%、60.71%(P<0.05).就5环HMW-PAHs去除率,H+J3处理显著高于H+J1处理,H+J2与H+J3之间差异不显著;针对6环HMW-PAHs去除率,H+J3处理显著高于H+J1、H+J2处理.从土壤酶活性来看,ZH-H2—环糊精2因素处理下土壤木质素过氧化物酶活性均显著高于其他处理,且与5环、6环芳烃去除率大体上呈现显著正相关关系.综合来看,ZH-H2(1 g/kg)—环糊精(5 g/kg)处理是高效修复5~6环芳烃的优势组合.
Research on cyclodextrin enhanced Fusarium remediation of soil contaminated ecological environment with HMW-PAHs
The soil contaminated by high cyclic aromatic hydrocarbons(HMW-PAHs)in coal mine area was taken as the research object.Remediation effects of Fusarium ZH-H2 treat-ment,cyclodextrin treatment and a combination of the two on 6 monomeric PAHs in 5-ring and 6-ring contaminated soil were studied by adding cyclodextrin and inoculating Fusarium ZH-H2.The results showed that the removal rates of 5-ring and 6-ring PAHs by Fusarium were 14.37%-22.85%,and the removal rate of 6 HMW-PAHs monomers were the highest under different doses of cyclodextrin treatment(J1,J2,J3),J2 treatment(1 g/kg)was the highest,which was 13.51%-28.43%,and in the ZH-H2-cyclodextrin treatment,H+J3 treatment had the best removal effect on 6 HMW-PAHs monomers(BbF,BkF,BaP,DbA,InP,BghiP)and the total amount,with removal rates of 24.61%,25.15%,23.14%,34.18%,25.76%,23.46%and 25.29%,respectively,which were 71.33%,66.68%,28.94%,49.58%,44.94%,55.88%and 60.71%higher than those of ZH-H2 single treat-ment(P<0.05).The removal rate of 5-ring HMW-PAHs was significantly higher in H+J3 treatment than in H+J1 treatment,and there was no significant difference between H+J2 and H+J3,while in terms of removal rate of 6-ring HMW-PAHs,H+J3 treatment was sig-nificantly higher than that in H+J1 and H+J2 treatments.From the perspective of soil en-zyme activity,the activity of soil lignin peroxide under ZH-H2-cyclodextrin treatment was significantly higher than that of other treatments,and there was a significant positive corre-lation with the removal rate of 5-ring and 6-ring HMW-PAHs.On the whole,ZH-H2(1 g/kg)-cyclodextrin(5 g/kg)treatment was an advantageous combination for efficient treat-ment of 5-6 ring HMW-PAHs.

cyclodextrinFusariumHMW-PAHsremoval rateenzymatic activities

张萌、祁明月、张光伟、高佳圆、李家辉、王小敏、宁国辉、杨志新

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河北农业大学资源与环境科学学院,河北保定 071000

省部共建华北作物改良与调控国家重点实验室,河北保定 071000

环糊精 镰刀菌 高环多环芳烃 去除率 酶活性

河北省教育厅重点项目华北作物改良与调控国家重点实验室项目中央引导地方科技发展资金项目现代农业产业技术体系建设项目河北省创新团队建设项目

ZD2021057NCCIR2022ZZ-9226Z7301GHBCT2024140209

2024

林业与生态科学
河北农业大学

林业与生态科学

CSTPCD
影响因子:0.299
ISSN:2096-4749
年,卷(期):2024.39(2)
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