Research on cyclodextrin enhanced Fusarium remediation of soil contaminated ecological environment with HMW-PAHs
The soil contaminated by high cyclic aromatic hydrocarbons(HMW-PAHs)in coal mine area was taken as the research object.Remediation effects of Fusarium ZH-H2 treat-ment,cyclodextrin treatment and a combination of the two on 6 monomeric PAHs in 5-ring and 6-ring contaminated soil were studied by adding cyclodextrin and inoculating Fusarium ZH-H2.The results showed that the removal rates of 5-ring and 6-ring PAHs by Fusarium were 14.37%-22.85%,and the removal rate of 6 HMW-PAHs monomers were the highest under different doses of cyclodextrin treatment(J1,J2,J3),J2 treatment(1 g/kg)was the highest,which was 13.51%-28.43%,and in the ZH-H2-cyclodextrin treatment,H+J3 treatment had the best removal effect on 6 HMW-PAHs monomers(BbF,BkF,BaP,DbA,InP,BghiP)and the total amount,with removal rates of 24.61%,25.15%,23.14%,34.18%,25.76%,23.46%and 25.29%,respectively,which were 71.33%,66.68%,28.94%,49.58%,44.94%,55.88%and 60.71%higher than those of ZH-H2 single treat-ment(P<0.05).The removal rate of 5-ring HMW-PAHs was significantly higher in H+J3 treatment than in H+J1 treatment,and there was no significant difference between H+J2 and H+J3,while in terms of removal rate of 6-ring HMW-PAHs,H+J3 treatment was sig-nificantly higher than that in H+J1 and H+J2 treatments.From the perspective of soil en-zyme activity,the activity of soil lignin peroxide under ZH-H2-cyclodextrin treatment was significantly higher than that of other treatments,and there was a significant positive corre-lation with the removal rate of 5-ring and 6-ring HMW-PAHs.On the whole,ZH-H2(1 g/kg)-cyclodextrin(5 g/kg)treatment was an advantageous combination for efficient treat-ment of 5-6 ring HMW-PAHs.