Diversity Analysis of Symbiotic Bacteria in Three Species of Water Mites
In order to investigate the composition and diversity of symbiotic bacteria in three water mite species,Hydrachna(Rhabdohydrachna)crassa,Lebertia(Lebertia)fimbriata and Hygrobates(Hygrobates)bravisterus,high-throughput 16S rDNA sequencing was employed to compare and analyze the symbiotic bacterial communities in these water mites.Following rarefaction,a total of 20,844 sequences were obtained from the sequencing data of the three water mite species,with 34,39,and 233 operational taxonomic units(OTUs)identified for Hydrachna(Rhabdohydrachna)crassa,Lebertia(Lebertia)fimbriata,and Hygrobates(Hygrobates)bravisterus,respectively.The highest diversity of symbiotic bacteria was observed in H.(H.)bravisterus,while the greatest concentration of dominant bacteria was noted in H.(R.)crassa.At the phylum and class taxonomic levels,the most abundant symbiotic bacteria were found to belong to the phylum Proteobacteria and class Alphaproteobacteria across all three water mites.At lower taxonomic levels,the most abundant dominant bacteria in H.(R.)crassa and L.(L.)fimbriata were identified as belonging to the order Rickettsiales,family Rickettsiaceae,and genus Rickettsia.In contrast,the most abundant dominant bacteria in H.(H.)bravisterus were associated with the order Rhizobiales.Regarding the composition and abundance of symbiotic bacteria,similarities were observed between H.(R.)crassa and L.(L.)fimbriata,and distinct differences were noted in H.(H.)bravisterus compared to the other two species.The study indicates that there are differences in the diversity of symbiotic bacteria among the three species of water mites,and different living enviroments may be the primary cause affecting these differences.
water mitehigh-throughput sequencingsymbiotic bacteria16S rDNAdiversity
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NSTL
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