Effect of honeysuckle polysaccharides on the biological behavior of triple-negative breast cancer cells
Objective To investigate the effects of Honeysuckle polysaccharide on the prolifera-tion,apoptosis and migration of triple-negative breast cancer cells.Methods The triple-negative hu-man breast cancer cell line MDA-MB-231 was divided into a blank control group and four intervention groups(A,B,C,D)treated with different concentrations of Honeysuckle polysaccharide(200 mg/mL,400 mg/mL,600 mg/mL,800 mg/mL).The control group received no treatment,and the inter-vention groups were treated with 20 μL of the respective concentrations of Honeysuckle polysaccha-ride.After 24 hours and 48 hours,the cell proliferation was detected using MTT assay,cell migration was detected using a scratch assay,apoptosis was detected using TUNEL staining,and the expression levels of Bcl-2 and Bax mRNA were detected using RT-PCR.Results The proliferation rate,migration distance,and expression level of Bcl-2 mRNA in the intervention groups were significantly lower than those in the control group(P<0.05).The apoptosis rate and expression level of Bax mRNA in the intervention groups were significantly higher than those in the control group(P<0.05).With in-creasing concentration ofHoneysuckle polysaccharide,the proliferation rate,migration distance and expression level of Bcl-2 mRNA decreased,while the apoptosis rate and expression level of Bax mRNA increased(P<0.05).The proliferation rate and expression level of Bcl-2 mRNA in the intervention groups were significantly lower at 48 hours than at 24 hours(P<0.05).The apoptosis rate and ex-pression level of Bax mRNA were significantly higher at 48 hours than at 24 hours(P<0.05).Conclu-sion Honeysuckle polysaccharide inhibits the proliferation and migration of triple-negative breast cancer cells and promotes their apoptosis.This effect is closely related to the regulation of Bcl-2/Bax apoptotic pathway.
Honeysuckle polysaccharideTriple negative breast cancerProliferationApop-tosisMigrationBiological behavior
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