Exploring the mechanism of Shenling Baizhu San in the treatment of tuberculosis based on network phar-macology and molecular docking
Objective To predict the potential mechanism of the treatment of pulmonary tubercu-losis by Shenling Baizhu San through network pharmacology and molecular docking technology.Meth-ods Use the TCMSP database to obtain the active components of Shenling Baizhu San and their corre-sponding target genes.Screen the relevant targets of tuberculosis disease by Genecards and OMIM da-tabases,and select the common target genes of drugs and diseases.The protein interaction network map(PPI)was constructed using the STRING database.The"disease-target-effective component"network map was obtained using Cytoscape 3.8.0 software.The effective target genes were imported into the Metascape database and microbial information platform for GO and KEGG enrichment visual-ization analysis.The main active components were docked with key genes using Maestro 12.9 software.Results A total of 189 active components and 365 protein targets were screened,of which 151 target genes may be related to the treatment of pulmonary tuberculosis.Quercetin,luteolin,kaempferol,12-senecioyl-2 E,8 E,10 E-atractylentriol and Chrysanthemin were the top 5 active components in Degree value ranking.PTGS2,AR,NOS2,MAPK14 and ADRB2 were key genes,and the active components had strong binding abilities with key target genes.Conclusion This study preliminarily predicts that the prescription Shenling Baizhu San can treat pulmonary tuberculosis through multiple targets and multiple pathways,providing a basis for further mechanism research in the future.
万方数据
维普
