Effect of syringin combined with gemcitabine on the growth of pancreatic cancer cells
Objective To investigate the effect of syringin combined with gemcitabine on the growth of pancreatic cancer cells and predict its possible molecular mechanism.Methods(1)Using the GDSC database,the pancreatic cancer cell with the strongest resistance to gemcitabine was screened out from three different types of pancreatic cancer cells.(2)MTT assay was used to detect the effects of syringin,gemcitabine,and their combination on the survival rate of human pancreatic cancer cell line AsPC-1,and the half-maximal inhibitory concentration(IC50)of syringin,gemcit-abine,on AsPC-1 cells was obtained.(3)Trypan blue assay was further used to verify the effect of combined drug use on AsPC-1 cells.(4)Genes related to syringin and gemcitabine were searched through the CTD database,and a Venn diagram was drawn to identify common genes.(5)The Kap-lan-Meierplotter database was used to perform survival analysis on the selected common genes to iden-tify genes closely related to the combined use of syringin and gemcitabine.Results(1)Among the three pancreatic cancer cell lines,AsPC-1 cells exhibited the strongest resistance to gemcitabine.(2)Syringin,gemcitabine,and their combination all inhibited the survival rate of AsPC-1 cells:the IC50 value of syringin was(41.76±0.37)mmol/L,the IC50 value of gemcitabine was(0.01±0.07)mmol/L.(3)Genes related to syringin and gemcitabine included TNF,MPO,NFKBIA,IFNG,IL-4,IL-13,RELA,and IL-6.(4)Genes related to the effect of combined drug use included TNF,IL-13,and IFNG.Conclusion The combined application of syringin and gemcitabine may inhibit the growth of pancreatic cancer cells through TNF,IL-13,and IFNG genes.
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