摘要
目的 分析没食子-甘草凝胶调节牙周炎局部Th17/Tregs的影响,阐明其在牙周免疫调节及牙周炎治疗中的意义.方法 将SD大鼠随机等量分为对照组、牙周炎组及观察组,采用结扎法建立病理模型.观察组给予自制没食子甘草凝胶进行干预.评价各组实验动物牙龈出血、牙齿松动以及牙槽骨吸收情况.分析各组牙龈及外周血中Th17/Tregs的比例差异及IL-17、IL-10以及TGF-β的表达水平.结果 与对照组比较,牙周炎组牙龈出血、牙齿松动及牙槽骨吸收水平均升高,观察组仅牙齿松动升高(P＜0.05).牙周炎组与对照组比较,牙龈中Th17比例升高、Tregs下降(P＜0.05),观察组未表现出此现象.牙周炎组与对照组比较IL-17升高,而IL-10、TGF-β下降(P＜0.05),而观察组仅IL-10以及TGF-β的mRNA低于对照组(P＜0.05),余差异无统计学意义(P＞0.05).结论 没食子-甘草凝胶有效缓解牙龈出血、牙齿松动、牙槽骨吸收及牙龈Th17比例异常,对牙周炎有治疗效果.
Abstract
Objective To analyze the effect of gallnut-glycyrrhizae gel on regulating the local Th17/Tregs balance in periodontitis and elucidate its significance in periodontal immune regulation and periodontitis treatment.Methods SD rats were randomly divided into a control group,a peri-odontitis group,and an observation group in equal proportions.A pathological model was established using the ligation method.The observation group was intervened with self-made gallnut-glycyrrhizae gel.The gingival bleeding,tooth mobility,and alveolar bone resorption were evaluated in each group.The differences in the ratio of Th17/Tregs and the expression levels of IL-17,IL-10,and TGF-β in gingival tissue and peripheral blood were analyzed.Results Compared with the control group,the periodontitis group showed increased levels of gingival bleeding,tooth mobility,and alve-olar bone resorption,while only tooth mobility increased in the observation group(P＜0.05).Com-pared with the control group,the periodontitis group exhibited an increase in the proportion of Th17 and a decrease in Tregs in gingival tissue(P＜0.05),which was not observed in the observation group.The periodontitis group had elevated IL-17 and decreased IL-10 and TGF-β levels compared to the control group(P＜0.05),while the observation group only showed lower mRNA levels of IL-10 and TGF-β compared to the control group(P＜0.05),with no significant differences in the other in-dicators(P＞0.05).Conclusion Gallnut-glycyrrhizae gel effectively alleviates gingival bleeding,tooth mobility,alveolar bone resorption,and abnormal Th17 proportion in gingival tissue,demon-strating a therapeutic effect on periodontitis.
基金项目
吉林省预算内基本建设资金-产业技术研究与开发专项(2022C041-8)
万方数据