Gallnut-glycyrrhizae gel on local inflammation and Th17/Tregs balance in experimental periodontitis model
Objective To analyze the effect of gallnut-glycyrrhizae gel on regulating the local Th17/Tregs balance in periodontitis and elucidate its significance in periodontal immune regulation and periodontitis treatment.Methods SD rats were randomly divided into a control group,a peri-odontitis group,and an observation group in equal proportions.A pathological model was established using the ligation method.The observation group was intervened with self-made gallnut-glycyrrhizae gel.The gingival bleeding,tooth mobility,and alveolar bone resorption were evaluated in each group.The differences in the ratio of Th17/Tregs and the expression levels of IL-17,IL-10,and TGF-β in gingival tissue and peripheral blood were analyzed.Results Compared with the control group,the periodontitis group showed increased levels of gingival bleeding,tooth mobility,and alve-olar bone resorption,while only tooth mobility increased in the observation group(P＜0.05).Com-pared with the control group,the periodontitis group exhibited an increase in the proportion of Th17 and a decrease in Tregs in gingival tissue(P＜0.05),which was not observed in the observation group.The periodontitis group had elevated IL-17 and decreased IL-10 and TGF-β levels compared to the control group(P＜0.05),while the observation group only showed lower mRNA levels of IL-10 and TGF-β compared to the control group(P＜0.05),with no significant differences in the other in-dicators(P＞0.05).Conclusion Gallnut-glycyrrhizae gel effectively alleviates gingival bleeding,tooth mobility,alveolar bone resorption,and abnormal Th17 proportion in gingival tissue,demon-strating a therapeutic effect on periodontitis.
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