Analysis of the Progeny from Wide Cross T.timopheevi and Avena fatua L.var.Portugal Using SRAP Technique
The hybrid progenies from wide cross T.timopheevi×Avena fatua L.var.Portugal were analyzed using SRAP markers to prove their truth and understand their characterizations in the present study.Among 22 primer pairs,20 yielded the polymorphic product between the both parents,and the average polymorphic ratio was 73.81%.Four primer pairs (me4-em1,me3-em5,me4-em3 and me3-em3) produced the specific markers for either parent in the F_3 line,indicating that the F_3 line contained both parental homologues of some chromosomes,and were true intergeneric hybrid origin.The F_3 line lost some parental SRAP markers,and the markers lost from T.timopheevi were far less than those from the wild oat;meanwhile,some novel SRAP markers were generated.These DNA sequence changes might contribute to the rapid evolution of the newly formed allopolyploid and allow the divergent genomes to act in harmony.
国家科技期刊平台
NSTL
万方数据
维普
