Identification of Microsatellite Markers Associated with Growth Rate Traits in Hucho taimen
22 microsatellite markers were used to analyze the growth rate related traits of 176 individuals from Fl of random mating Hucho taimen,including body weight,body length,body height,body width,head length,head width and interorbital width. The results showed that of the 67 alleles detected in total, the numbers of alleles ranged from 2 to 6 per locus with the fragments of 109 to 452 bp,and the mean observed heteozygosity and the mean expected heteozygosity were 0.583 5 and 0. 564 0, respectively. Deviations from Hardy-Weinberg equilibrium (HWE) occurred at 22 loci in different degrees. The normal distribution of 7 traits was analyzed using SPSS software and the results indicated that all the seven traits had a characteristic of continuous variation belonging to the typical quantitative traits or polygenic inheritance traits,and conformed to the normal distribution. Meanwhile,GLM linear model was constructed between the 22 microsatellite markers and the growth rate related traits, and the markers significantly correlative to the traits were performed multiple comparison based on the genotypes. The results revealed that of 22 microsatellite loci,there were 11 markers significantly correlative to one trait at least,and the minimum number of markers related to body length was 4 and the maximum number of markers related to body height and body width was 7. Through the multiple comparisons, it was found that there was significant difference of genotypes from the same trait in every locus,and there was also difference among the mean value of the different markers. The screening of these markers could provide some basis for the further study on molecular marker-assisted breeding.
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