以不同生态型甘蓝抗枯萎病高代自交系 R4－P1和感枯萎病自交系 R2－P2组配得到的142个 F2单株作为构图群体，利用 AFLP、SSR、SRAP 这3种分子标记技术对该群体进行遗传连锁分析，构建了一张含有9个连锁群、240个标记位点的高密度甘蓝分子遗传连锁图谱（LOD≥4），其包含162个 AFLP 标记、52个 SSR 标记和26个 SRAP 标记，标记间平均图距3．6 cM，覆盖864．4 cM。该图谱涉及标记种类多，是目前发表的覆盖基因组较全面的结球甘蓝亚种内遗传连锁图谱，可供参考的 SSR 标记数量多，将为后期甘蓝枯萎病抗性基因的定位及克隆搭建良好平台，同时为结球甘蓝重要农艺性状的 QTL 定位及分子标记辅助选育新品种奠定基础。
Construction of a Genetic Linkage Map in Cabbage Based on AFLP,SSR and SRAP Markers
A F2 population consisting of 142 individual plants derived from a cross between fusarium wilt resistant cabbage self line R4-P1 and fusarium wilt susceptible line R2-P2 combining with AFLP ,SSR and SRAP markers were applied to construct a high density genetic linkage map of Brassica oleracea.In total,240 markers including 162 AFLP markers,52 SSR markers and 26 SRAP markers were integrated into 9 groups(LOD≥4).It covered 864.4 cM,with the average interval of 3.6 cM.It is a relatively high dense map involving more kinds of markers especially more refer -ence SSR markers.It is also one of the most comprehensive intrasific genetic linkage map of covering whole genome of cabbage.It will build a good platform for mapping and cloning of the fusarium wilt resistant gene in cabbage ,and fur-thermore,lay the foundation for mapping important agronomic traits for marker assistant breeding .
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