Optimization of Chromosome Mounting Technique and Karyotype Analysis of Culinary Rhubarb
In order to better understand the information about cytology of culinary rhubarb ,provide information support for genetic breeding of culinary rhubarb .The protocol of different sampling time ,pretreatments methods ,dis-sociating time on chromosome observation from culinary rhubarb root tips was studied and karyotype of two cultivars ( RAMB and RO) was analyzed .The observation showed that root tips collecting at 8:00 am,pretreated in ice water mixture for 3 to 5min,dissocated in 1 mol/L HCl at 60℃for 15 min,dyed with modified carbolic acid-magenta for 3 to 5 min could achieve the optimal experimental results .Karyotype analysis of two cultivars of culinary rhubarb showed that the chromosome number was 44,with karyotype formula being 2n=4x=44=36m+8sm and 2n=4x=44=32m+12sm,respectively.The results demonstrated that the asymmetry indexes were 57.46% and 58.60%, respectively.The karyotype from the two cultivars of culinary rhubarb was 1B type and was symmetrical ,suggesting that culinary rhubarb was relatively primitive forms .
国家科技期刊平台
NSTL
万方数据
维普
