首页|FT/TFL 类基因在山葡萄雌雄花中的表达分析

FT/TFL 类基因在山葡萄雌雄花中的表达分析

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为了研究FT/TFL类基因山葡萄雌花和雄花中的表达模式,以山葡萄3个雄花品系和3个雌花品系为材料,根据GenBank上已经发表的3个葡萄FT/TFL类基因设计引物,利用实时荧光定量PCR技术研究VvFT、VvTFL1A和VvTFL1B这3个基因在山葡萄雌花和雄花中的表达。3个基因在山葡萄雌花和雄花中都有表达。 VvFT基因在雌花中的表达量显著高于雄花。而VvTFL1A和VvTFL1B这2个基因在雄花中的表达量略高于雌花。 VvFT基因的表达高峰出现在花序刚开始展露期(5月14日);而VvTFL1A和VvTFL1B表达高峰出现在芽体膨大期(4月30日)。结果表明,这两类基因在山葡萄花发育过程中作用时间不同。 FT类基因可能参与花的前期调控,而TFL类基因在花器官发育中起作用。
FT/TFL Expression Pattern in Male and Female Floral of Vitis amurensis Rupr
To study FT/TFL genes expression pattern in female and male floral of V itis amurensis Rupr.,three male and three female strains were used to study the expression of three FT/TFL genes (VvFT,VvTFL1A and VvTFL1B) by Real-time PCR technology.The three genes expressed not only in female but also in male floral .The relative expression of VvFT gene in female were significantly higher than in male floral ,while the relative expression of VvTFL1A and VvTFL1B genes in male flowers slightly higher than in female floral .The peak value of VvFT gene expression appeared at inflorescence show stage (May 14);while VvTFL1A and VvTFL1B expression appeared one peak value at bud intumescence stage ( April 30 ) .It was suggested that FT genes may be involved in regulation of early flowers,and TFL genes played a role in floral organ development .

Vitis amurensis Rupr.Real-time PCRFT/TFL gene

徐维华、郑秋玲、刘万好、沙玉芬、范书亭、唐美玲

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山东省烟台市农业科学研究院,山东 烟台 265200

中国农业科学院 左家特产研究所,吉林 左家 132019

山葡萄 实时荧光定量PCR FT/TFL基因

现代产业技术体系专项

CARS-30

2014

华北农学报
河北,北京,天津,山西,河南,内蒙古六省市农科院农学会

华北农学报

CSTPCDCSCD北大核心
影响因子:1.067
ISSN:1000-7091
年,卷(期):2014.(z1)
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