Characterization and Expression Pattern of Myosin Light Chain 2 Gene from Monochamus alternatus
In order to elucidate the muscle contraction mechanism and related gene expression pattern of Monochamus alternatus,the myosin light chain 2 gene from Monochamus alternatus, called MaMLC-2 ( GenBank:KM371003 ) was cloned by using cDNA library construction .The cloned cDNA of MaMLC-2 was 882 bp in length , which contains a 618 bp open reading frame ( ORF) encoding 205 amino acids with a calculated molecular mass of 22.26 kDa and an isoelectric point of 4.66.MaMLC-2 showed the highest homology with Tribolium castaneum (96%) ,and has amino acid sequence homology with the myosin light chain 2 from 8 species of insects such as Gr-yllotalpa orientalis ( 85%-88%) .M.alternatus and T.castaneum on the same branch in the phylogenetic tree based on insect myosin light chain 2 amino acids .MaMLC-2 was hydrophilic ,which contains 2 EF-hand functional domains ,and both of the domains are comprised of 29 amino acids .The first EF-hand functional domain can bind Ca2+.There was a Ca 2+binding site in the first EF-hand domain .SWISS-MODEL prediction results showed that MaMLC-2 has 2 helix-loop-helix structures .The relative expression levels of MaMLC-2 detected by RT-qPCR indi-cated that the expression levels of MaMLC-2 in pupae and larvae were higher than that in adults ,and the expression level in head of adults was the highest ,nevertheless,the expression level in abdomen of adults was the lowest .The MaMLC-2 were prominently and differently expressed in all of the adult parts ( P<0 .05 ) .
国家科技期刊平台
NSTL
万方数据
维普
