Fine Mapping of a Narrow Leaf Mutant vrnl11 in Mungbean
The identification of leaf shape mutant and genes controlling leaf shape can not only provide germ-plasm resources for genetic improvement of leaf shape,but also help to analyze the genetic regulation mechanism of leaf development.vrnl11 was identified from an EMS induced Wankelu 3(WK3)mutant library.Progeny populations derived from vrnl11/WK3 and vrnl11/Zhonglü 1 were used for genetic analysis,and the segregation pattern of differ-ent phenotypic plants in F2 populations was determined by x2 test.Two F2 populations constructed by crossing vrnl11 with Zhonglu 1 and Zhonglu 5 were used as mapping populations.Fine mapping for vrnl11 was completed by using BSA sequencing technology and map-based cloning strategy.Phenotype identification results showed that,compared with wild-type WK3,the leaf width and leaf area of vrnl11 decreased by 25.7％and 21.7％,respectively.Genetic analysis showed the narrow leaf phenotype of vrnl11 was controlled by a single recessive nuclear gene.BSA sequen-cing analysis located the mutation site within a 4.7 Mb interval from 15.0 Mb to the chromosomal end on chromo-some 11.vrnl11 was finally located in the 186.5 kb interval between the markers nl-61 and nl-46 by using these newly developed polymorphic molecular markers.The mapping interval contained 9 predicted genes.These results provide a theoretical basis for cloning vrnl11 and understanding the molecular regulation mechanism of leaf develop-ment in mungbean.
国家科技期刊平台
NSTL
万方数据
维普
