Genome-wide Identification of TCP Gene Family in Jasminum sambac and Expression Analysis Involved in Flower Development and Pollen-pistil Interaction
To explore the function of the TCP gene family in the growth and development of Jasminum sambac,bioinformatics methods were used to identify and analyze the whole genome of J.sambac TCP(JsTCP)gene family.Moreover,the expression levels of TCP gene family in different stages of flower development and pollen-stigma inter-action were analyzed.A total of 27 TCP gene family members were identified from the whole genome of J.sambac,named JsTCP1-JsTCP25.The protein contained 208-539 amino acid residues,with molecular weight of 22.95-56.96 ku and isoelectric point of 5.70-9.97,and all of them were unstable hydrophilic proteins.The subcellular localization prediction showed that all JsTCPs were located in the nucleus.JsTCPs were unevenly distributed on 10 chromosomes.Gene structure analysis showed that JsTCPs had 1-5 exons and 0-4 introns.Protein conserved motifs and phylogenetic analysis showed that JsTCPs contained conserved TCP domain,and they were divided into two sub-classes:Class Ⅰ and Class Ⅱ.Promoter of JsTCPs contained cis-acting elements related to plant hormone response,stress response,and growth and development.The expression pattern analysis showed that 24 JsTCPs were expressed at different stages of flower development,and 22 genes were expressed in pollen-stigma interactions.In conclusion,27 TCP gene family members were identified from J.sambac,and it found that they expressed specifically at differ-ent stages of flower development and different stages after pollination.
国家科技期刊平台
NSTL
万方数据
维普
