Identification and Analysis on the Resistance to Stripe Rust Candidate Gene of TraesCS2A01G070700 from Wheat Cultivar Annong 1687
To investigate the genetic mechanism behind Annong 1687's ability to resist stripe rust,expedite the dissemination of Annong 1687 wheat variety,offer guidance for enhancing the genetics of novel wheat varieties,and mitigate the damage inflicted by wheat stripe rust on Chinese wheat production.This study employed Annong 1687(Annong 1687 is a semi-winter wheat type with resistance to the less significant physiological species CYR32.It was developed through the crossing of Annong 1106 and Xinong 822)with its sister lines,and parents.The wheat 55K SNP microarray was used to scan the entire genome of Annong 1687 as well as parents and sister lines.Concurrent-ly,Annong 1687 and sister lines were inoculated and characterized employing the physiological race CYR32.The re-sistance to stripe rust grades was noted at the adult stage of the plant,and the resulting combined inoculation and field phenotypes were evaluated for stripe rust resistance of the wheat lines.The study revealed that Annong 1687 and five sister lines(lines 24,26,28,30 and 140)exhibited moderate resistance to stripe rust,while two sister lines(lines 89 and 105)displayed moderate susceptibility to this disease.Based on genomic differential data comparing Annong 1687 to its sister strains,there was evidence of enriched differential SNP loci in the 31-37 Mb segment of chromosome 2A's short arm,indicating possible presence of a gene or genes responsible for the result.To eliminate any potential interference from other stripe rust resistance genes,we verified the known stripe rust resistance genes present on the parental and chromosome 2A.Our findings indicated the possibility of a new stripe rust resistance gene in the 31-37 Mb interval of the short arm of chromosome 2A in Annong 1687.Experimental and bioinformatic analyses demonstrated that the TraesCS2A01 G070700 gene exhibited disparity in the NBS structural domain of re-sistant and susceptible lines within this interval.Additionally,susceptible lines possessed three missense mutations within the NBS structural domain.It was hypothesised that TraesCS2A01 G070700 represented a candidate gene for resistance to stripe rust in Annong 1687.
国家科技期刊平台
NSTL
万方数据
维普
