首页|50个小麦品种抗叶锈基因鉴定

50个小麦品种抗叶锈基因鉴定

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选育抗性品种是小麦叶锈病防治举措中最经济、可行的方法。为进一步挖掘抗病基因,选取河南、河北、山东等8个省小麦产区50个小麦品种。首先在苗期将16个叶锈菌生理小种(THFS、TGTS、THJS、FHKT、FGJN、KHKS、FCJQ、RFKS、THFM、MHGT、KHGS、KBGT、FHGT、PHHT、FHJT、FCJT)接种在36份小麦抗叶锈病近等基因系材料以及50个供试小麦品种上。因各菌种带有不同毒力,可根据表现型的差异,再将已知抗病基因紧密连锁的特异性分子标记与之结合分析,进而推测50份小麦材料中可能含有的抗叶锈病基因。通过基因推导、分子标记以及系谱分析综合鉴定抗锈性基因,结果表明,在50个品种中共检测出9个(Lr1、Lr2c、Lr10、Lr16、Lr26、Lr34、Lr37、Lr45和Lr46)已知抗叶锈性基因和少量未知基因。含有Lr1基因的有淄麦12等22个品种;含有Lr2c基因的有鲁麦14等10个品种;含有Lr10基因的只有莱州9361 一个品种;含有Lr16基因的有科农199等25个品种;含有Lr26基因的有徐州24等15个品种;含有Lr34基因的有宝麦3号和京冬8号;含有Lr37基因的有淄麦12、鹤0927和荷9946;含有Lr45基因的有连麦2号等11个品种;含有Lr46基因的有山农19等38个品种。
Identification of Leaf Rust Resistance Genes in 50 Wheat Cultivars
Breeding resistant varieties is the most economical and feasible method to control wheat leaf rust.In order to further explore the resistance genes,50 wheat varieties were selected in this study,including wheat varieties from Shan-dong,Henan,Hebei,Shandong and other 8 provinces.Firstly,16 physiological races of leaf rust fungus(THFS,TGTS,THJS,FHKT,FGJN,KHKS,FCJQ,RFKS,THFM,MHGT,KHGS,KBGT,FHGT,PHHT,FHJT,FCJT)were inoculated to 36 vector cultivars containing known leaf rust resistance genes,and 50 wheat varieties tested during the seedling stage.Due to the different virulence of each strain,specific molecular markers closely linked to known disease resistance genes can be combined and analyzed based on the differences in phenotype,and it can be inferred that 50 wheat materials may contain leaf rust resistance genes.The leaf rust resistance genes were identified by genetic deduction,molecular markers and pedigree analysis.The results showed that a total of 9 known resistance genes(Lr1,Lr2c,Lr10,Lr16,Lr26,Lr34,Lr37,Lr45 and Lr46)and a few unknown genes were detected in 50 cultivars.There were 22 varieties including Zimai 12 that contained the Lr1 gene;there were 10 varieties including Lumai 14 that contained the Lr2c gene;Laizhou 9361 only con-tained the Lr10 gene;there were 25 varieties including Kenong 199 that contained the Lr16 gene;there were 15 varieties including Xuzhou 24 that contained the Lr26 gene;Baomai 3 and Jingdong 8 contained the Lr34 gene;The Lr37 gene was contained in Zimai 12,He 0927,and He 9946;there were 11 varieties including Lianmai 2 that contained the Lr45 gene;there were 38 varieties including Shannong 19 that contained the Lr46 gene.

WheatLeaf rustGene derivationLeaf rust resistance gene

赵欣雨、康占海、王羽、李艳艳、高维芳、李星

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河北农业大学植物保护学院,河北保定 071001

河北农业大学食品科技学院,河北保定 071001

小麦 叶锈病 基因推导 抗叶锈病基因

国家自然科学基金引进留学人员资助项目

32001890C20230107

2024

华北农学报
河北,北京,天津,山西,河南,内蒙古六省市农科院农学会

华北农学报

CSTPCD北大核心
影响因子:1.067
ISSN:1000-7091
年,卷(期):2024.39(1)
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