Analysis of Differentially Expressed Protein of M239 in Response to Verticillium Wilt Pathogen Infection
In order to explore the mechanism of plant in response to Verticillium wilt pathogen infection,Dali wild eggplant(M239)was took as the material.By the artificial inoculation of Verticillium dahliae,four key physio-logical indicators of M239 root were determined at 0,6,12,24,and 48 h after inoculation with V.dahliae,which namely the peroxidase(POD)activity,catalase(CAT)activity,phenylalanine ammonilyase(PAL)activity and the soluble protein(SP)content.The result showed that 12 and 24 h after inoculation with V.dahliae were determined as the proper sampling time points for proteome analysis.On this basis,iTRAQ technique was used to analyze the protein changes of M239 root after inoculation with V.dahliae.Compared with CK,463 differentially expressed pro-teins(DEPs)were found in M239 root at 12 h after inoculation with V.dahliae,among which 305 DEPs were down-regulated and 158 were up-regulated.At 24 h after inoculation with V.dahliae in M239,456 DEPs were in-duced to express by pathogens,including 296 down-regulated and 160 up-regulated proteins.These proteins were mainly enriched in carbon metabolism,glyoxylate and dicarboxylic metabolism,biosynthesis of secondary metabo-lites,pyruvate metabolism,and metabolic pathways.Compared with the reported Verticillium wilt resistant materi-als,it was found that the number of proteins and metabolic pathways involved in the defense response of M239 in the process of responding to the infection of V.dahliae was less.Therefore,it failed to form an effective defense network,and finally manifested as susceptible to the disease.
Wild eggplantVerticillium wiltVerticillium dahliaeIsobaric tag for relative and absolute quanti-tation(iTRAQ)Differentially expressed proteins
国家科技期刊平台
NSTL
万方数据
维普
