大理野茄M239响应黄萎病病菌侵染的差异表达蛋白分析
Analysis of Differentially Expressed Protein of M239 in Response to Verticillium Wilt Pathogen Infection
蔚亚楠 1黎志彬 2龚亚菊 2鲍锐 2桂敏 2杜光辉 3刘家迅 2吴丽艳2
作者信息
- 1. 云南省农业科学院园艺作物研究所,云南昆明 650205;云南大学资源植物研究院,云南昆明 650500
- 2. 云南省农业科学院园艺作物研究所,云南昆明 650205
- 3. 云南大学资源植物研究院,云南昆明 650500
- 折叠
摘要
为了探索植物响应黄萎病病原菌侵染的作用机制,以黄萎病高感野茄材料大理野茄(M239)为研究对象,通过人工接种黄萎病病原菌大丽轮枝菌的方法,首先测定M239在接种大丽轮枝菌后0,6,12,24,48 h的根部4个关键生理指标,即过氧化物酶(POD)、过氧化氢酶(CAT)、苯丙氨酸解氨酶(PAL)的活性和可溶性蛋白(SP)含量的变化.结果确定接种大丽轮枝菌后12,24 h为蛋白组分析取样的关键时间点;在此基础上,利用iTRAQ技术分析M239在接种大丽轮枝菌后根部蛋白的变化.结果发现,与清水接种(CK)相比,M239接种大丽轮枝菌后12 h有463个差异表达蛋白(DEPs),其中305个下调,158个上调;接种后24 h,有456个DEPs被病原菌诱导表达,包括296个下调,160个上调.这些蛋白主要富集在碳代谢、乙醛酸和二羧酸代谢、次生代谢物的生物合成、丙酮酸代谢、新陈代谢等途径.通过与已报道的黄萎病抗性材料的研究结果进行对比分析发现,M239在响应大丽轮枝菌的侵染过程中,参与防御响应的蛋白数量和代谢通路较少,未能形成有效的防御网络,最终表现为感病.
Abstract
In order to explore the mechanism of plant in response to Verticillium wilt pathogen infection,Dali wild eggplant(M239)was took as the material.By the artificial inoculation of Verticillium dahliae,four key physio-logical indicators of M239 root were determined at 0,6,12,24,and 48 h after inoculation with V.dahliae,which namely the peroxidase(POD)activity,catalase(CAT)activity,phenylalanine ammonilyase(PAL)activity and the soluble protein(SP)content.The result showed that 12 and 24 h after inoculation with V.dahliae were determined as the proper sampling time points for proteome analysis.On this basis,iTRAQ technique was used to analyze the protein changes of M239 root after inoculation with V.dahliae.Compared with CK,463 differentially expressed pro-teins(DEPs)were found in M239 root at 12 h after inoculation with V.dahliae,among which 305 DEPs were down-regulated and 158 were up-regulated.At 24 h after inoculation with V.dahliae in M239,456 DEPs were in-duced to express by pathogens,including 296 down-regulated and 160 up-regulated proteins.These proteins were mainly enriched in carbon metabolism,glyoxylate and dicarboxylic metabolism,biosynthesis of secondary metabo-lites,pyruvate metabolism,and metabolic pathways.Compared with the reported Verticillium wilt resistant materi-als,it was found that the number of proteins and metabolic pathways involved in the defense response of M239 in the process of responding to the infection of V.dahliae was less.Therefore,it failed to form an effective defense network,and finally manifested as susceptible to the disease.
关键词
野茄/黄萎病/大丽轮枝菌/iTRAQ技术/差异表达蛋白Key words
Wild eggplant/Verticillium wilt/Verticillium dahliae/Isobaric tag for relative and absolute quanti-tation(iTRAQ)/Differentially expressed proteins引用本文复制引用
基金项目
云南省重大科技专项计划(202102AE090005)
云南省重大科技专项计划(202102AE090051)
云南省兴滇英才支持计划青年人才专项(吴丽艳)()
出版年
2024
国家科技期刊平台
NSTL
维普
万方数据