Effect of lncRNA2099 on Proliferation and Differentiation of Porcine Preadipocytes
To investigate the effect of lncRNA2099 on the proliferation and differentiation of porcine preadipo-cytes,the qRT-PCR was used to detect the expression level of lncRNA2099 in heart,liver,spleen,lung,kidney,back muscle,back fat and proliferation and differentiation stages of porcine preadipocyte.The nucleoplasmic separation technique was used to determine the expression location of lncRNA2099 in adipocyte.The eukaryotic expression vec-tor pcDNA3.1-lncRNA2099 was constructed and qRT-PCR was used to detect its impact on the expression of marker genes related to adipocyte proliferation and differentiation after over expression in adipocyte.The results showed that lncRNA2099 was highly expressed in kidney and back fat,but not in back muscle.The nucleoplasmic localization re-sults showed that lncRNA2099 was expressed in both nucleus and cytoplasm;the expression levels of proliferation marker gene P21 and lipogenic differentiation marker gene LPL significantly increased after overexpression of ln-cRNA2099 in adipocytes.lncRNA2099 may act as a transcriptional regulator to inhibit proliferation and promote lipo-genic differentiation in porcine preadipocytes.
国家科技期刊平台
NSTL
万方数据
维普
