The Effect of Zearalenone on the Growth of Bovine Mammary Epithelial Cells and the Expression of Genes Related to Milk Fat Synthesis
This study aims to explore the effects of different concentrations of zearalenone(ZEN)on the growth of bovine mammary alveolar cell-T(MAC-T)and the expression of genes related to milk fat synthesis.First-ly,MAC-T cells were treated with different doses of ZEN for 36 hours,and the number of cells was counted using a hemocytometer.Then,cells were stained and analyzed for apoptosis and necrosis to determine the appropriate dose of ZEN.Next,acommercial detection kit was used to examine the impact of ZEN on reactive oxygen species(ROS)and mitochondria number in MAC-T cells.Finally,using Real-time Quantitative PCR(RT-qPCR)technology,the influence of ZEN on proliferation,apoptosis,oxidative stress,and milk fat synthesis-related gene expression in MAC-T cells was analyzed.The results showed that low doses of ZEN(0.01-1.00 μmol/L)tended to promote MAC-T growth,whereas high doses of ZEN(5.00-10.00 μmol/L)significantly reduced the number of cells,0.10 μmol/LZEN had no obvious effect on ROS and mitochondria number in MAC-T,but 10.00 μmol/L ZEN no-tably elevated ROS levels and decreased mitochondria number.RT-qPCR results indicated that 0.10 μmol/L ZEN significantly promoted the expression of proliferation genes(CDK1,CCND2),antioxidant genes(DHODH,GPX4),and anti-apoptotic genes(BCL-2),but also increased the expression of apoptotic genes(CAS-3,BAX).Whereas,10.00 μmol/L ZEN significantly inhibited the expression of proliferation genes(PCNA,CDK1,CCND2),antioxi-dant genes(DHODH,GPX4,AIFM2),and anti-apoptotic genes(BCL-2),while significantly promoting the expres-sion of apoptotic genes(CAS-3,BAX).Notably,0.10 μmol/L ZEN obviously promoted the expression of milk fat synthesis-related genes(PPARy,FASN,JAK-2),but 10.00 μmol/L ZEN significantly suppressed these genes.The aforementioned results suggested different effects of ZEN concentrations on MAC-T cells:0.10 μmol/L ZEN could promote MAC-T cell growth and milk fat synthesis-related gene expression while also inducing the expression of ap-optotic genes.In contrast,10.00 μmol/L ZEN induces oxidative stress in MAC-T cells,reduces mitochondria num-ber,inhibits the expression of genes associated with proliferation,antioxidant properties,anti-apoptotic properties,and milk fat synthesis,while promoting the expression of apoptotic genes,leading to cell apoptosis.
国家科技期刊平台
NSTL
万方数据
维普
