Screening and Analysis of LncRNA Related to Muscle Development in Longissimus Dorsi Muscle of Different Sex Tan Sheep
This study aims to analyze the effects of LncRNA on muscle development of male and female Tan sheep from the gene level,because muscle growth and development is an important indicator of economic benefit in breeding industry,and skeletal muscle development is significantly correlated with meat production performance.In or-der to preliminarily reveal the molecular mechanism affecting the differences in muscle development,transcriptome se-quencing analysis was conducted to screen the LncRNA related to sheep muscle development.Full transcriptome se-quencing(RNA-seq)and analysis were performed on longissimus dorsi muscle tissues of 3 male and female sheeps(8 months old),so as to screen out LncRNAs that may be related to muscle development of Tan sheep.The results showed that among the 8 513 LncRNAs were identified,45 intergroup differentially expressed LncRNAs were selected,of which 18 were up-regulated and 27 down-regulated.Prediction of co_location target gene of DELncRNA and GO functional enrichment analysis showed that target genes were significantly enriched in 268 GO terms,including somato-secreting cell differentiation,typical Wnt receptor signaling pathway involved in negative regulation of apoptosis,posi-tive regulation of growth hormone secretion,and cAMP-mediated signaling.KEGG pathway enrichment analysis showed that DEGs was enriched in AMPK,NF-KB,PI3K-AKT,MAPK,cAMP,FOXO and other signaling pathways.Five Ln-cRNAs,namely,TCONS_00017263,TCONS_00147966,TCONS_00163484,TCONS_00079802,and TCONS_00079803,which may be involved in muscle development,were further screened.Real-time Fluorescence Quantitative PCR was performed on 6 randomly selected DELs,and the expression trend was consistent with the trend of transcriptome se-quencing,which further verified the accuracy of the sequencing results.These DELncRNAs obtained in the study may cause the differences in muscle growth and development of Tan sheep between males and females,and these related LncRNAs may provide more information for regulating muscle development and provide scientific basis for molecular breeding of meat performance of Tan sheep of different genders in Ningxia in the future.
Tan sheepLncRNARNA-seq technologyMuscle developmentqRT-PCR
国家科技期刊平台
NSTL
万方数据
维普
