Cloning and Expression Characteristic Analysis of PlSPL1 Gene in Paeonia lactiflora Pall.
To investigate the nature and function of PlSPL1(SPL)gene of Paeonia lactiflora,further elucidate the commonalities and characteristic differences of the PlSPL1 gene in different species,and explore the role of Pl-SPL1 in the degree of stem straightening in Paeonia lactiflora.The full-length sequence of the PlSPL1 gene was ob-tained by RACE technology using the stalk of herbaceous peony Hongfeng as the research material,and the structural,physicochemical properties,and phylogenetic relationships of PlSPL1 were analyzed and predicted using bioinformatics software,then,the expression level of PlSPL1 in different developmental stages of herbaceous peony stems was ana-lyzed using qRT-PCR technology,and subcellular localization analysis of the protein was conducted using laser confo-cal microscopy technology.The results showed that the open reading frame of the PlSPL1 gene was 3000 bp,encoding 999 amino acids.The protein had a molecular formula of C4869 H7682 N1406 O1497 S43,a molecular weight of 111.25 ku,a theoretical isoelectric point of 6.26,encoding a hydrophilic unstable acidic protein,phosphorylation modification pre-dominantly on serine,no signal peptide,with transmembrane structure,and had a secondary structure consisting mainly of random coil.Phylogenetic tree analysis revealed that the PlSPL1 protein was most closely related to peony,followed by a closer relationship with grape;protein sequence comparison analysis revealed that the PlSPL1 protein had a con-served SBP domain which was unique to SPL transcription factor family.The relative expression analysis found that PlSPL1 gradually showed a decreasing trend with stem development,indicating that PlSPL1 was negatively regula-ting the stem development of herbaceous peony and it was hypothesised that PlSPL1 played important roles in de-gree of stem straightness;and the subcellular localisation showed that the PlSPL1 protein was localised in the nucle-us.The above results indicate that PlSPL1 is participated in the stalk development of herbaceous peony.
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