Cloning of Coding Region of Bovine ISG20 Gene and Its Expression Analysis in Receptivity Formation of Endometrial Epithelial Cell
In order to explore the expression and function of interferon-stimulated exonuclease gene 20(ISG20)in the formation of receptivity in beef cattle endometrial epithelial cells.The CDS region of the beef cattle ISG20 was ob-tained by RT-PCR combined with sequencing.The sequence analysis of the ISG20 and its encoded proteins was per-formed using bioinformatics online tools.The expression of the ISG20 and its related genes was detected by qRT-PCR in the formation of receptivity in beef cattle endometrial epithelial cells.The results showed that the length of CDS re-gion of the ISG20 was 516 bp,which could encode a single protein consisting of 176 amino acids with no transmem-brane domain and unstable basic hydrophilicity.The protein contained the DEDD(or DnaQ-like)exonuclease super-family domain,interacted with 10 proteins,including ISG15 and MX2,and functioned mainly in the cytoplasm.In ad-dition,the qRT-PCR results showed that compared with the control group,the expression of the ISG20 and its related ISG15 and MX2 was both significantly up-regulated in the formation of receptivity in beef cattle endometrial epithelial cells induced by progesterone(P4)-co-interferon-tau(IFN-τ).ISG20 may be involved in the formation of receptivity in beef cattle endometrial epithelial cells and play a role in biological processes such as early pregnancy.
国家科技期刊平台
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