肉牛ISG20基因编码区的克隆及其在子宫内膜上皮细胞容受态形成中的表达分析
Cloning of Coding Region of Bovine ISG20 Gene and Its Expression Analysis in Receptivity Formation of Endometrial Epithelial Cell
姚妮 1罗仍卓么 1包斌武 1杨易 2王兴平1
作者信息
- 1. 宁夏大学 动物科技学院,宁夏 银川 750021
- 2. 宁夏大学 生命科学学院,宁夏 银川 750021
- 折叠
摘要
为探究干扰素刺激外切酶基因20(ISG20)在肉牛子宫内膜上皮细胞容受态形成中的表达及功能分析.采用RT-PCR结合测序技术获得肉牛ISG20 基因的CDS区,利用生物信息学在线工具进行了ISG20 基因及其所编码的蛋白质的序列分析,并利用qRT-PCR检测了ISG20 基因及其相关基因在肉牛子宫内膜上皮细胞容受态形成中的表达量.结果显示,ISG20 基因的CDS区长度为516 bp,可编码一条由176 个氨基酸组成的无跨膜结构域且不稳定的碱性亲水性单体蛋白质.该蛋白质含有1 个DEDD 核酸外切酶超家族结构域,与ISG15 和MX2 等10 种蛋白质相互作用,主要在细胞质中发挥作用.此外,qRT-PCR结果表明,与对照组相比,ISG20 基因及其相关的ISG15 和MX2 在孕酮(P4)联合干扰素-tau(IFN-τ)诱导的肉牛子宫内膜上皮细胞容受态形成中的表达量均显著上调.ISG20 可能参与肉牛子宫内膜上皮细胞容受态的形成,并在早期妊娠等生物学过程中发挥作用.
Abstract
In order to explore the expression and function of interferon-stimulated exonuclease gene 20(ISG20)in the formation of receptivity in beef cattle endometrial epithelial cells.The CDS region of the beef cattle ISG20 was ob-tained by RT-PCR combined with sequencing.The sequence analysis of the ISG20 and its encoded proteins was per-formed using bioinformatics online tools.The expression of the ISG20 and its related genes was detected by qRT-PCR in the formation of receptivity in beef cattle endometrial epithelial cells.The results showed that the length of CDS re-gion of the ISG20 was 516 bp,which could encode a single protein consisting of 176 amino acids with no transmem-brane domain and unstable basic hydrophilicity.The protein contained the DEDD(or DnaQ-like)exonuclease super-family domain,interacted with 10 proteins,including ISG15 and MX2,and functioned mainly in the cytoplasm.In ad-dition,the qRT-PCR results showed that compared with the control group,the expression of the ISG20 and its related ISG15 and MX2 was both significantly up-regulated in the formation of receptivity in beef cattle endometrial epithelial cells induced by progesterone(P4)-co-interferon-tau(IFN-τ).ISG20 may be involved in the formation of receptivity in beef cattle endometrial epithelial cells and play a role in biological processes such as early pregnancy.
关键词
肉牛/ISG20/子宫内膜上皮细胞/细胞容受性/生物信息学分析Key words
Beef cattle/ISG20/Endometrial epithelial cells/Cell receptivity/Bioinformatics analysis引用本文复制引用
基金项目
宁夏回族自治区重点研发计划(2021BEF02029)
宁夏回族自治区重点研发项目(引才专项)(2020BEB04006)
出版年
2024
国家科技期刊平台
NSTL
万方数据