Cloning and Expression Pattern Analysis of ZmPAP26b Gene in Maize under Drought Stress
Drought stress has a serious effect on the growth and development of maize,which leads to a de-crease of maize yield.Purple acid phosphatase is a phospholipase protein involved in many physiological and bio-chemical functions of plants.In order to further study the role of purple acid phosphatase family genes in the process of stress resistance of maize,this paper explored the response mode of ZmPAP26b gene under drought stress,and Real-time fluorescence Quantitavive analysis was used to analyze the relative gene expression in different maize in-breeding lines under simulated drought conditions;ZmPAP26b(GenBank:NC_050104.1)was cloned from maize,and PAP genes in Zea mays,Arabidopsis thaliana,Oryza sativa L.,Triticum aestivum L.,Sorghum bicolor and Brachypodium distachyon were identified and bioinformatic analysis was performed.Meanwhile,prokaryotic overex-pression strains were constructed for functional verification.The results showed that the expression of this gene de-creased in drought tolerant materials and increased in drought sensitive materials under drought stress.The CDS length of this gene was 1 431 bp,encoding 476 amino acids.A total of 228 PAP genes were found in six species,di-vided into 4 subfamilies by phylogenetic analysis.The 19 PAP genes in maize were distributed on 9 chromosomes and had similar conserved domains.Analysis of promoter cis-acting elements showed that they contained elements responding to drought and hormones.Prokaryotic expression experiments showed that the growth of strains containing the recombinant plasmid pET28a-ZmPAP26b was inhibited compared with non-loaded strains under 10％PEG-6000 and 15％PEG-6000 simulated drought stress.In summary,it is speculated that ZmPAP26b is negatively regu-lated under drought stress.
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