QTL Mapping of Silk Coloring Characteristics of Anthocyanins in Maize
Silk color is an important agronomic trait for determining distinctiveness and uniformity of maize varie-ty.In order to analyze the genetic mechanism of silk coloring characteristics of anthocyanins in maize,a doubled hap-loid(DH)population with 213 lines derived from green silk inbred line WL134 and purple silk inbred line D7 was used for QTL mapping analysis under the environment of 2022 and 2023,respectively.The results showed that there were significant differences in silk coloring characteristics of anthocyanins among different lines and years,the herita-bility was 0.864.A total of 9 QTLs were detected in two years.These QTLs with phenotypic variation explained(PVE)ranging from 4.83%to 9.26%were detected on chromosomes 2,3,5,6,8,and 9 of maize.A stable and repeatable site qSC5 located between 19.15 Mb and 19.80 Mb was detected on chromosome 5 in two-year data.The LOD scores of the major QTLs were 4.65 and 5.76 in 2022 and 2023 respectively,with phenotypic variation explained(PVE)of 7.22%and 7.17%.It was a new site for regulating silk coloring characteristics of anthocyanins compared with previ-ous studies in maize.Based on SNP markers on both sides of qSC5,90.91%of the genotypes in extreme purple silk DH lines were CCCC,while only 44.00%of the genotypes in green silk DH lines.This marker was significantly corre-lated with silk color in maize and might link to key genes regulating silk color characteristics of anthocyanins.