Identification and Candidate Gene Mining of Stem Lodging Resistance in Brassica napus
In order to promote the cultivation of lodging resistant varieties of Brassica napus and explore the ge-netic resources of lodging resistance in the stem of Brassica napus,this study used four lodging resistant and three eas-ily lodging resistant germiplasm as materials,and measured the content of five components,including cellulose,hemi-cellulose,total pectin,protopectin,and lignin,in the lower stem during the flowering period.Transcriptome sequencing analysis was performed on the lower stem of YLS0084(lodging resistant)and YLS1691(easily lodging prone).The re-sults showed that the average content of cellulose and total pectin in the anti toppling material,significantly higher than materials prone to lodging;transcriptome analysis revealed a total of 7 397 differentially expressed genes with upregulation and 9 438 downregulation,which were enriched in pathways such as carbohydrate metabolism,transla-tion,amino acid metabolism,and signal transduction;nine genes related to lodging resistance in rapeseed(BnaA01G0071800ZS,BnaA01G0175700ZS,BnaA01G0205800ZS,BnaA03G0404800ZS,BnaA03G0517200ZS,BnaA05G0431400ZS,BnaA07G0056300ZS,BnaA09G0031300ZS,and BnaC05G0128400ZS)were validated through qRT-PCR,and were significantly upregulated in YLS0084.The results of this study demonstrates that the content of cellulose and total pectin in the stem of Brassica napus has a positive effect on lodging resistance and pro-vides important genetic resources for lodging resistance in Brassica napus.
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