Cloning and Functional Prediction of Castor PIP5K2 Gene
In plants,the PIP5K2 gene of the phosphatidylinositol 4,5-bisphosphate PIP5K gene family plays a key role in regulating plant growth.To investigate the function of the PIP5K2 gene in castor gene cloning,bioinfor-matics analysis,and expression analysis of the castor PIP5K2 gene were conducted.The results showed that a gene fragment of 2 136 bp in length was obtained through PCR using castor cDNA as the template.Bioinformatics analysis of the protein sequence encoded by this gene determined that the PIP5K2 gene encoded a protein consisting of 672 amino acids,with a pI value of 6.74 and a molecular weight of 76.47 ku.The protein's average hydrophilicity was-0.636,classifying it as a hydrophilic protein.Its secondary structure included α-helices,β-turns,extended strands,and random coils.Based on the prediction results,the tertiary structure of the PIP5K2 protein was consistent with its secondary structure,and shared a high degree of homology with the Jatropha curcas.The relative expression levels of the PIP5K2 gene were generally high in the five-leaf stage of the female,marker female and bisexual inflo-rescence types,and were generally low in the main stem panicle flowering stage.The highest relative expression lev-el was observed in the five-leaf stage of the marker female inflorescence,while the lowest relative expression level was observed in the main stem panicle flowering stage of the bisexual inflorescence.The highest relative expression level was approximately 80 times higher than the lowest relative expression level.Among the three inflorescence types,the relative expression level of the PIP5K2 gene was similar in the four-leaf stage.However,compared to dif-ferent growth stages within each inflorescence type,the expression level of the PIP5K2 gene was significantly higher in the four-leaf stage than in the flowering stage.Based on these results,it can be inferred that the PIP5K2 gene may regulate the mid-stage growth of castor plants and have some correlation with plant dwarfing.
国家科技期刊平台
NSTL
万方数据
