Isolation and Functional Analysis of Garlic Calmodulin Genes AsCML15 and AsCML42 under Osmotic Stress
Calmodulin-like proteins(CMLs),one of the Ca2+receptors in plants,are involved in the process of plant growth and development,as well as adaptation to environmental changes.To understand the sequence charac-teristics of garlic CMLs and their responses to osmotic stress,AsCML1 5 and AsCML42 genes from garlic variety Can-gshan siliuban were cloned,and their expression patterns under drought and salt stress conditions were determined.The results showed that the open reading frame of AsCML15 and AsCML42 genes were 498 and 543 bp in length,re-spectively,encoding 165 and 180 amino acid residues.AsCML15 and AsCML42 harbored four and three EF-hand domains,respectively.AsCML42 was closer to Arabidopsis AtCML42 and AtCML43 in evolutionary relationship,whereas AsCML15 was more closely related to Arabidopsis AtCML15 and AtCML16.Real-time Quantitative PCR technology showed that AsCML15 and AsCML42 were expressed in bulbs,leaves,and roots,and these two genes can be induced by 200 mmol/L NaCl and 15％PEG6000.The AsCML15 and AsCML42 genes may be involved in the process of garlic resisting salt and drought stress,and their biological functions can be further identified.
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